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Wisconsin Lab Testing Services
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WVRG Test Request Form: Print (HTML) or Download (PDF)
Test Pricing Information
Sample collection and shipping information
List of Diagnostic Tests Performed
2000 Rapid Human Herpesvirus Six (HHV-6) Culture andAntigenemia
Methodology:
Shell vial culture. Detects both active HHV-6 A and HHV-6 B infection, but does not differentiate between them.
Performed: Tuesday, Wednesday, Thursday, Friday
Reported: Within 5 days after receipt in lab
Specimen Required:
Collect: Whole blood: 7-10 ml in ACD (acid citrate dextrose).
Bone marrow: 2-3 ml in sodium heparin.
Bronchoalveolar lavage: 2-3 ml in sterile, leak-proof container.
Transport: ACD tube and bone marrow at room temperature.
Bronchoalveolar lavage refrigerated (wet ice).
Ship Monday-Thursday.
Unacceptable Conditions:
Specimen received frozen.
Specimen received greater than 48 hours from collection.
Serum or plasma received instead of whole blood.
Stability: Whole blood and bone marrow: Room temperature, 48 hours.
Bronchoalveolar lavage: Refrigerated, 48 hours.
Remarks:
Only one ACD tube necessary for the Rapid HHV-6 Culture and PCR
testing.
Transport as whole blood.
Reference Value: Negative.
Result is qualitative. Negative or Positive.
Interpretive Data:
Negative result suggests, but does not prove, the absence of active HHV-6 infection. Positive result is consistent with active HHV-6 infection.
1100 Human Herpesvirus Six (HHV-6) DNA Nested PCR
Methodology:
Polymerase Chain Reaction. Enhanced sensitivity of nested PCR for the detection of active infection. Detects and differentiates HHV-6 A and HHV-6 B.
Performed: Monday - Friday
Reported: Within 4 days of receipt in lab.
Specimen Required:
Collect: CSF or one 7 to 10 ml ACD (acid citrate dextrose) tube.
Transport: 1 ml CSF or plasma (ACD) in a sterile, leak-proof container, frozen (dry ice).
Or ship ACD tube as whole blood at room temperature.
Unacceptable Conditions:
Whole blood received frozen.
Whole blood received greater than 48 hours from collection.
Stability:
Plasma and CSF: Room temperature, 4 hours. Refrigerator, 3 days. Frozen, 1 year.
Whole blood: Room temperature, 48 hours.
Reference Value: Negative
1200 Epstein-Barr Virus (EBV) DNA Nested PCR
Methodology:
Polymerase Chain Reaction. Enhanced sensitivity of nested PCR for the detection of active infection.
Performed: Monday - Friday
Reported: Within 4 days of receipt in lab.
Specimen Required:
Collect: One 7 to 10 ml ACD (acid citrate dextrose) tube.
Transport: Plasma (ACD) in a sterile, leak-proof container, frozen (dry ice).
Or ship ACD tube as whole blood at
room temperature.
Unacceptable Conditions:
Whole blood received frozen.
Whole blood received greater than 48 hours from collection.
Stability:
Plasma: Room temperature, 4 hours. Refrigerator, 3 days. Frozen, 1 year.
Reference Value: Negative
3000 Rapid Human Cytomegalovirus (HCMV) Culture and Antigenemia
Methodology:
Shell vial culture. Detects active HCMV infections by immunofluorescent staining with a HCMV specific monoclonal antibody.
Performed: Tuesday, Wednesday, Thursday, Friday
Reported: Within 5 days upon receipt in lab
Specimen Required:
Collect: Whole blood: 7-10 ml in ACD (acid citrate dextrose).
Bone marrow: 2-3 ml in sodium heparin.
Bronchoalveolar lavage: 2-3 ml in sterile, leak-proof container.
Transport: ACD tube and bone marrow at room temperature.
Bronchoalveolar lavage refrigerated (wet ice).
Ship Monday - Thursday.
Unacceptable Conditions:
Specimen received frozen.
Specimen received greater than 48 hours from collection.
Serum or plasma received instead of whole blood.
Stability:
Whole blood and bone marrow: Room temperature, 48 hours.
Bronchoalveolar lavage: Refrigerated, 48 hours.
Remarks:
Only one ACD tube necessary for the Rapid HCMV Culture, Rapid HHV-6 Culture and PCR testing. Transport
as whole blood.
Reference Value: Negative
Result is qualitative. Negative or Positive.
Interpretive Data:
Negative result suggests but does not prove the absence of active HCMV infection.
Positive result is consistent with active HCMV infection.
1400 Human Cytomegalovirus (HCMV) DNA Nested PCR
Methodology:
Polymerase Chain Reaction. Enhanced sensitivity of nested PCR for the detection of active infection.
Performed: Monday - Friday
Reported: Within 4 days upon receipt in lab.
Specimen Required:
Collect: CSF or one 7 to 10 ml ACD (acid citrate dextrose) tube.
Transport: 1 ml CSF or plasma (ACD) in a sterile, leak-proof container, frozen (dry ice).
Or ship ACD tube as whole blood at room temperature.
Unacceptable Conditions:
Whole blood received frozen.
Whole blood received greater than 48 hours from collection.
Stability:
Plasma and CSF: Room temperature, 4 hours. Refrigerator, 3 days. Frozen, 1 year.
Whole blood: Room temperature, 48 hours.
Reference Value: Negative
1300 Human Herpesvirus Seven (HHV-7) DNA PCR
Methodology:
Polymerase Chain Reaction. Enhanced sensitivity of nested PCR for the detection of active infection.
Performed: Monday - Friday
Reported: Within 4 days upon receipt in lab.
Specimen Required:
Collect: One 7 to 10 ml ACD (acid citrate dextrose) tube.
Transport: Plasma (ACD) in a sterile, leak-proof container, frozen (dry ice). Or ship ACD tube as whole blood at
room temperature.
Unacceptable Conditions:
Whole blood received frozen. Whole blood received greater than 48 hours from collection.
Stability:
Plasma: Room temperature, 4 hours. Refrigerator, 3 days. Frozen, 1 year.
Reference Value: Negative
Additional instructions
All specimens should be labeled with the patient's name and collection date.
Ship specimen with a completed WVRG Test Request Form.
Ship specimens for testing, Monday through Thursday, to the following address:
Wisconsin Viral Research Group
10437 Innovation Drive
Suite 321
Milwaukee, WI 53226
Call (414) 774-8612 for a specimen kit with instructions for the submission of blood samples.
PCR tests use a commercial kit or reagent that has not been approved or cleared by the FDA.
The performance characteristics were determined by WVRG.